| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.057592 |
| Chromosome: | chromosome 2 |
| Location: | 7075343 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389986 | (1 of 17) IPR029069 - HotDog domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTCAGGCGAGCGTGGACCGCCCTCCCACTTGACCAGCAAGTCATTACT |
| Internal bar code: | AAATAATGAATGCAATTGCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3439 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTCCGCTTTGCATATGTG |
| Suggested primer 2: | GCACCGTCCAAAGACCAAAC |