| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.057598 |
| Chromosome: | chromosome 3 |
| Location: | 1678144 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g153050 | RWP1 | (1 of 1) IPR000104//IPR003035 - Antifreeze protein, type I // RWP-RK domain; RWP-RK transcription factor | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAATGCAATCGCGGGACTGGCCAGTCCCCACTTCGCAAAAAACAAAGC |
| Internal bar code: | CGAGTATCGGTTAAGATAGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2498 |
| LEAP-Seq percent confirming: | 86.7924 |
| LEAP-Seq n confirming: | 138 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 159 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGAAATATCAGCCCGCTCT |
| Suggested primer 2: | GCAATAAACCGTCGAAGGCC |