Insertion junction: CLIP2.057606_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.057606
Chromosome:chromosome 2
Location:6700343
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g387282 (1 of 1) PTHR13600//PTHR13600:SF22 - LEUCINE CARBOXYL METHYLTRANSFERASE // S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASES SUPERFAMILY PROTEIN 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCATTGTGGTGACACCCTGCCACCTTCGCTCCCGGCCTATCACTCCCGC
Internal bar code:CTGGTGCGGCGTTGATCATTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1385
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:48
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:GCACGCACATTTCGTTCCTT
Suggested primer 2:ATGTCCGATCGTCGAACCAG