| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.057614 |
| Chromosome: | chromosome 11 |
| Location: | 851640 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467645 | (1 of 2) PTHR10540:SF8 - COP9 SIGNALOSOME COMPLEX SUBUNIT 6 | 5'UTR | |
| Cre11.g801245 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCATGATACTCCGATGTCCAAATGTTCGATGTTACAAGCCGAGGAACAA |
| Internal bar code: | ATCGTAAAGTAGATTCGGCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1854 |
| LEAP-Seq percent confirming: | 89.5833 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAACTCCTGGCACGTACAA |
| Suggested primer 2: | TTTCTGTTCCGTGCCATCGA |