| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.057628 |
| Chromosome: | chromosome 12 |
| Location: | 4302461 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g518650 | BSD1,BHSD1 | (1 of 2) K07748 - sterol-4alpha-carboxylate 3-dehydrogenase (decarboxylating) (E1.1.1.170, NSDHL, ERG26); 3-beta hydroxysteroid dehydrogenase | 3'UTR |
| Cre12.g518700 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATTGCACAGTTCACGAGTACGATGTGCCTGCTATAAGGGTTGAAGCACG |
| Internal bar code: | CGTGCCCGGGGAAGATCGTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1319 |
| LEAP-Seq percent confirming: | 38.8889 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGCTTCTTGCTACATGTC |
| Suggested primer 2: | CAGCATGAAGGACGCGATTG |