Insertion junction: CLIP2.057630_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.057630
Chromosome:chromosome 16
Location:2254033
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre16.g658526 MNME1,TGD3,MNME (1 of 1) K03977 - GTP-binding protein (engA); Putative ABC transport system ATP-binding protein intron
Cre16.g801906 (1 of 1) PTHR24220//PTHR24220:SF441 - FAMILY NOT NAMED // PROTEIN TRIGALACTOSYLDIACYLGLYCEROL 3, CHLOROPLASTIC 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTATCCAATCTAACGATGGCGCAGCAAGGCTGTGATGCACACGGTTTTG
Internal bar code:AACCTCCGATTTTGTATGGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:737
LEAP-Seq percent confirming:25.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GGTTGTTCTCTGGGCGGTTA
Suggested primer 2:GCGGCATCTCCTCCTACATC