| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.057635 |
| Chromosome: | chromosome 10 |
| Location: | 4495079 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g451050 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGTGCGGTGTGTGTAAGGCAGCCTTTCATTGCCTGCGCCACCCGCCTA |
| Internal bar code: | TCTGACGAGCACGGATGACCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 118 |
| LEAP-Seq percent confirming: | 28.3019 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGAGCTGTGCGAGTATGG |
| Suggested primer 2: | GTGCCTCATGACTGACAGCT |