Insertion junction: CLIP2.057647_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.057647
Chromosome:plastome
Location:187451
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802331 2716963,ChreCp066,psbC (1 of 1) K02705 - photosystem II CP43 chlorophyll apoprotein (psbC); photosystem II 43 kDa protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTACTAACCCAACAACAAATGCTGCTGTTATTTTTGGTTACCTTGTAAA
Internal bar code:TGGGGCCCCTGTCCTGTTGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:67
LEAP-Seq percent confirming:9.33333
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:68
LEAP-Seq n unique pos:75

Suggested primers for confirmation by PCR

Suggested primer 1:TTTTCGAAACCAGCAGCAGC
Suggested primer 2:GGCCGTGACCAAGAAACAAC