| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.057659 |
| Chromosome: | chromosome 3 |
| Location: | 6605032 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g194800 | (1 of 1) K14399 - polyribonucleotide 5'-hydroxyl-kinase (CLP1, HERB) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTACGGCGCAGATTCTCTGCGGGTCATGTCAGCCCCCCATGTCCCTTGGA |
| Internal bar code: | CAAAACAGACTTAGGAGCGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4034 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 91 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 91 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCACAGCTTATGGGGTGT |
| Suggested primer 2: | AGTGCGTGTGTGCTAATGGA |