| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.057662 |
| Chromosome: | chromosome 2 |
| Location: | 4657332 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g107650 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGAATGACTCTTGCACCACAGGCTCCTGTCAGTCCTGTGAGCAGGAAC |
| Internal bar code: | CGTGTAATGGGGTTAATGGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4035 |
| LEAP-Seq percent confirming: | 28.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAAGCAACGCTGAAGAAA |
| Suggested primer 2: | CAGTTCAGTCGGACACGTCA |