| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.057669 |
| Chromosome: | chromosome 12 |
| Location: | 3365031 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g498300 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGATGGGAGCCAAAGCGCCTCTACAGAAAGGCCTCTCCACAGCGTCAAT |
| Internal bar code: | CTTTGCCGGGTTAGCAGTAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1993 |
| LEAP-Seq percent confirming: | 40.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACCTGTTGGCTGTCAAAG |
| Suggested primer 2: | GCTGAATGCGCTTCATCAGG |