| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.057673 |
| Chromosome: | chromosome 2 |
| Location: | 596560 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g077350 | HDH1 | Histidinol dehydrogenase; (1 of 1) 1.1.1.23 - Histidinol dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGGTGCCGCTCTGTGCGTGAAGCAATTGCACATGCCTCACCCCTTGCT |
| Internal bar code: | CCGCTGGTTCCGCCGATGGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 822 |
| LEAP-Seq percent confirming: | 93.9394 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTTCTCCCCGCACAGAAC |
| Suggested primer 2: | GCATTCCTCGCATTCTGCTG |