Insertion junction: CLIP2.057681_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.057681
Chromosome:chromosome 2
Location:1400405
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre02.g083300 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGTCACCACGGTCTCCGCGGCCTCCGCCGCCCTCCCGGTCCCCGCGGTC
Internal bar code:AACACAATGTAGGCCGCCAACA

Confirmation - LEAP-Seq

LEAP-Seq distance:491
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGTTCTTGCCATGTTGCG
Suggested primer 2:CGCGTCAATTTAGCTCCAGC