| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.057693 |
| Chromosome: | chromosome 12 |
| Location: | 5282271 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g528050 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTGCAAGGGAGAGCCACCTTTTCTTGTTCAAAGTGTAAGGGTGCGCTG |
| Internal bar code: | GTGCTGCCTATATCCCGAGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1302 |
| LEAP-Seq percent confirming: | 95.2381 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGGTGTCTCGCAAACTCC |
| Suggested primer 2: | CTGCCGGACAAGTGGTATGT |