| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.057694 |
| Chromosome: | chromosome 14 |
| Location: | 1020247 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g614050 | (1 of 1) K16732 - protein regulator of cytokinesis 1 (PRC1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGAAGTCATCTTGGACGTGACCAGGTGTGGGGATCCCGAGGGAAAAGC |
| Internal bar code: | GTTTCTTGTGCTTCAGCTTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3000 |
| LEAP-Seq percent confirming: | 95.8333 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTCCTAGCAGCCCCATCT |
| Suggested primer 2: | AAGCTGACCTTCTCCATGCC |