| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.057695 |
| Chromosome: | chromosome 12 |
| Location: | 1214026 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g490250 | (1 of 1) PTHR12658//PTHR12658:SF0 - BETA-TUBULIN COFACTOR D // TUBULIN-SPECIFIC CHAPERONE D | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCAGGCTAGAGCTGCACCACGCGTTAAACTAGGCTGTCTTCGATTTCG |
| Internal bar code: | TTCATAATATTCGTTAGACCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1103 |
| LEAP-Seq percent confirming: | 59.5238 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTAGAGCTGCGGGTGAA |
| Suggested primer 2: | GGTCGTGAGGTTTGGCTGTA |