| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.057700 |
| Chromosome: | chromosome 8 |
| Location: | 102259 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358529 | Similar to Flagellar Associated Protein FAP157 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTCAGCACACTGCCGGGCTCAGCTAGCAGGCCGGCCCTATGCCCAAGG |
| Internal bar code: | GTACATCATCCAAGGGACTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 330 |
| LEAP-Seq percent confirming: | 18.4211 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTTCCTCCTTCCCCGTAG |
| Suggested primer 2: | ATTGATGGGGGCGCCTTAAA |