| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.057707 |
| Chromosome: | chromosome 10 |
| Location: | 3058152 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g801138 | (1 of 9) IPR011011//IPR013083 - Zinc finger, FYVE/PHD-type // Zinc finger, RING/FYVE/PHD-type | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGGGTTGTGTGTGCGCGTGTGAGTGCCTGTTTGCGGCGGGCGTGTAT |
| Internal bar code: | CCACGTCGCTTGGGTGAGGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5250 |
| LEAP-Seq percent confirming: | 50.7463 |
| LEAP-Seq n confirming: | 68 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 134 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGAGGTGTTGTTTCCGAG |
| Suggested primer 2: | CGGCAGCGATATGTCCATCT |