Insertion junction: CLIP2.057724_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.057724
Chromosome:plastome
Location:28308
Confidence (%):40
Locus systematic id Locus common name Defline Feature
CreCp.g802277 2717058,rpl14,ChreCp015 50S ribosomal protein L14; (1 of 1) PTHR11761//PTHR11761:SF13 - 50S/60S RIBOSOMAL PROTEIN L14/L23 // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATAAATATCGGCAGTTGGCAGGCAACAATAAATAAATTTGTCCACTAAA
Internal bar code:TCGATCTAGCAGGGCATTTTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:368
LEAP-Seq percent confirming:47.0588
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:TGCATCACCAATACCACGGT
Suggested primer 2:TATTCGTGCTCTTGGTGGCA