Insertion junction: CLIP2.057728_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.057728
Chromosome:chromosome 7
Location:2650595
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g330550 3'UTR
Cre07.g330600 (1 of 2) K01510 - apyrase (ENTPD1_3_8) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCAGTGGACTGTCACTGTCCGACTGGGGACATCCGAGGCTCTCTCGCA
Internal bar code:CCGAATAAACACGTTTGAGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2391
LEAP-Seq percent confirming:57.1429
LEAP-Seq n confirming:36
LEAP-Seq n nonconfirming:27
LEAP-Seq n unique pos:63

Suggested primers for confirmation by PCR

Suggested primer 1:GGCATTGAATACGCCAGCTG
Suggested primer 2:GTGGTTGTCAAGGTGGTGGA