| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.057728 |
| Chromosome: | chromosome 7 |
| Location: | 2650596 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g330550 | 3'UTR | ||
| Cre07.g330600 | (1 of 2) K01510 - apyrase (ENTPD1_3_8) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGTACATCGCGTTACAGCATGGCCACTGCGGCGCGCGTCCCTCGCCGC |
| Internal bar code: | ATCGGGCGGGAGGTGACGAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 333 |
| LEAP-Seq percent confirming: | 22.2222 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGTTGTCAAGGTGGTGGA |
| Suggested primer 2: | GGCATTGAATACGCCAGCTG |