| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.057771 |
| Chromosome: | chromosome 12 |
| Location: | 4916659 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g524100 | ABH1,CBP80 | mRNA cap-binding protein; (1 of 1) K12882 - nuclear cap-binding protein subunit 1 (NCBP1, CBP80) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGACGACTGTCCTACTACACCGGCCTTGCCACGGCCTTCAAACGACCG |
| Internal bar code: | GAGCTCCACGCCCACTCTTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 595 |
| LEAP-Seq percent confirming: | 79.3103 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGTGGGTGATGGTGTAGG |
| Suggested primer 2: | GATGTGGGGTTGAGGGTGAG |