| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.057778 |
| Chromosome: | chromosome 16 |
| Location: | 6672818 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g673300 | Chlamydomonas-specific family W protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCGTCATTGCCGGGGCCGCCGTCGACCCCAGCCTCCATGAAGTTTGTG |
| Internal bar code: | ATACCGTGCGCTCGGGGAATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 362 |
| LEAP-Seq percent confirming: | 60.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGTAACCCTTGCGGAACT |
| Suggested primer 2: | CCGACAAGCTCCAGTTGACT |