| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.057783 |
| Chromosome: | chromosome 10 |
| Location: | 2323558 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g434450 | NUOA9 | (1 of 1) K03953 - NADH dehydrogenase (ubiquinone) 1 alpha subcomplex subunit 9 (NDUFA9); NADH:ubiquinone oxidoreductase 39 kDa subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCAAACACAAAGCGACCCGCCTATCACAACCTCACCGCCCCGACGCGA |
| Internal bar code: | TGCTGTCTGTTGGCCTGAATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2278 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAGCCCTTCGACTCTGTG |
| Suggested primer 2: | AGCTCCCACCCGTTGTATTG |