Insertion junction: CLIP2.057799_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.057799
Chromosome:chromosome 17
Location:1974003
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g710300 PHC31 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 31 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAAGTCATAGTCTGTGAAACGGCTTGCCGTCATAATTGCCCGCGCCTACA
Internal bar code:TGTCGATGTCTGAATTGTAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1187
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:GTTTGCCCATAGATGCGCTG
Suggested primer 2:CGTACGTACGTCAGGAGCTC