| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.057866 |
| Chromosome: | chromosome 1 |
| Location: | 3601338 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g023500 | FAL2 | Similar to Flagellar Associated Protein FAP234 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGGGAGCCGGAGCCGGAGCCGCAGCCGCAGCCGGAGCCGGAGCCGCAG |
| Internal bar code: | AAGTATCACCTTGGGTACAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 493 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGACACTGCTGACACTGCT |
| Suggested primer 2: | CGCACCTCCAAAGTCGAGAT |