| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.057871 |
| Chromosome: | chromosome 6 |
| Location: | 574533 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g253300 | UBCH1 | (1 of 1) K05610 - ubiquitin carboxyl-terminal hydrolase L5 [EC:3.4.19.12] (UCHL5, UCH37) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAGCAAGTCGGGAGACGCGGGGTTATTGAGTGTCCGTTCTGCACCTGC |
| Internal bar code: | CCACGCATCAAAATCTCCGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2760 |
| LEAP-Seq percent confirming: | 97.8723 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 94 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGTCTGAGTCGTCATCGG |
| Suggested primer 2: | GTTTTTGGTCGGGGTCGTTG |