| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.057889 |
| Chromosome: | chromosome 17 |
| Location: | 3329270 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g722650 | 3'UTR | ||
| Cre17.g802066 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGACGCGACTCGAGCGCAAATGACCGGGACAGCCTTACAAATGTGGTAA |
| Internal bar code: | ACCGGGCACGGCAGGGAACAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6478 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCAGCAGCTGTTTGTTGG |
| Suggested primer 2: | GGGGAGGAGTTTGTGTAGGC |