| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.057896 |
| Chromosome: | chromosome 14 |
| Location: | 2452446 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g623800 | NAT6 | N-acetyltransferase; (1 of 1) PTHR23091//PTHR23091:SF66 - N-TERMINAL ACETYLTRANSFERASE // N-ALPHA-ACETYLTRANSFERASE 30 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACAGTCCGCTGGCGAAGGTCGGCCGTACCCAGATTGCTGTACATAGCAC |
| Internal bar code: | TGAATGAGAGATAACGAAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2886 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTAGTGTACACGGCAGT |
| Suggested primer 2: | ACACGGTTGACAAATGTGCG |