| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.057905 |
| Chromosome: | chromosome 14 |
| Location: | 2952092 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g627433 | (1 of 1) PF04142 - Nucleotide-sugar transporter (Nuc_sug_transp) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCGCTGTCTGCCCAGAGAGAGGTGAGGAGGGGCCCGGGCCCGGGCGC |
| Internal bar code: | CGCAGGTGTCGTAATGTAACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 145 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACATGGTAGCCGAATTGCG |
| Suggested primer 2: | GGTAAGGGGGAGGGGGTATT |