Insertion junction: CLIP2.057913_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.057913
Chromosome:chromosome 3
Location:3788885
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g169650 (1 of 8) 2.4.1.5 - Dextransucrase / Sucrose 6-glucosyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCTACACACACGCGCCCACATACACTTACACACAAACGCACACCAATGC
Internal bar code:TACTGTGATATGGGGATGAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1541
LEAP-Seq percent confirming:95.8333
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:CCACTTCAGCTTCGTGTCGG
Suggested primer 2:CCACCTGACACCACCTTCTC