Insertion junction: CLIP2.057913_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.057913
Chromosome:chromosome 3
Location:3788887
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g169650 (1 of 8) 2.4.1.5 - Dextransucrase / Sucrose 6-glucosyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCATGCGCTGACGGGCCTTTGACTGGTGGGAGGTGGAACAGCGGTCTCA
Internal bar code:TACTGTGATATGGGGATGAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:3028
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:82
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:82

Suggested primers for confirmation by PCR

Suggested primer 1:CCACCTGACACCACCTTCTC
Suggested primer 2:CCACTTCAGCTTCGTGTCGG