Insertion junction: CLIP2.057916_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.057916
Chromosome:chromosome 6
Location:1643142
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g261950 ANK11 (1 of 29) PF13637 - Ankyrin repeats (many copies) (Ank_4); Predicted protein with ankyrin repeats 5'UTR
Cre06.g262000 SNR5 SNARE-associated Golgi protein; (1 of 6) PF09335 - SNARE associated Golgi protein (SNARE_assoc) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAGGTCGTGGAGAGGTATGCGGCAGGTGATAGGACCGCGGCGTTTGAGC
Internal bar code:TCCGGATATCTTAATTACTAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:157
LEAP-Seq percent confirming:4.65116
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:41
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:CCATGTCCTGTCCCAATGCT
Suggested primer 2:GATAGCTCCCCTGTACGTGC