Insertion junction: CLIP2.057921_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.057921
Chromosome:chromosome 13
Location:2698841
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g581650 PRPL7,L12 (1 of 1) PF00542//PF16320 - Ribosomal protein L7/L12 C-terminal domain (Ribosomal_L12) // Ribosomal protein L7/L12 dimerisation domain (Ribosomal_L12_N); Chloroplast ribosomal protein L7/L12 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCTTCGGGCCTTAATTTTGGGGCCGCTTGCATGGGCGCCGTACCATTGC
Internal bar code:GCCTAAGTCCTTCTTTTTAAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2058
LEAP-Seq percent confirming:79.5455
LEAP-Seq n confirming:35
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:AAAGCGTGCATTGCCATGTT
Suggested primer 2:TTGCGGACCACCTTGTAGAC