| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.057922 |
| Chromosome: | chromosome 9 |
| Location: | 5698599 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g410400 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATCTTGGTAGCCTGCCGTCGCTCGCTAAAAATGACAGCAGCGATATCG |
| Internal bar code: | GTGGAACCTGAAGTCTAGACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3074 |
| LEAP-Seq percent confirming: | 32.1429 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTACTGTTGCTGCTGACAC |
| Suggested primer 2: | TTGAATGCAGCCGTTTGACG |