| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.057922 |
| Chromosome: | chromosome 9 |
| Location: | 5698602 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g410400 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGGTCTCCTGGACTCCACCCGACGAAACTGGGAAGCAAAGCGAGCCT |
| Internal bar code: | ACGGATAGCCACGGTCTTGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2144 |
| LEAP-Seq percent confirming: | 91.3043 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAATGCAGCCGTTTGACG |
| Suggested primer 2: | GCTACTGTTGCTGCTGACAC |