| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.057940 |
| Chromosome: | chromosome 4 |
| Location: | 389506 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217912 | (1 of 1) PTHR13035//PTHR13035:SF0 - UNCHARACTERIZED // PROTEIN N-TERMINAL GLUTAMINE AMIDOHYDROLASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGATGCATGGGGCTGCGCCTTTCCATGAGCTGCATCTGGGCCGCACAC |
| Internal bar code: | GGAGAAAAAGCACACATCAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1946 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCAGGAATGCCAGACCAC |
| Suggested primer 2: | CTGCCGCTCCCTAGTTCAAA |