| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.057976 |
| Chromosome: | chromosome 12 |
| Location: | 5078893 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g525600 | (1 of 8) IPR001810//IPR006553 - F-box domain // Leucine-rich repeat, cysteine-containing subtype | outside_mRNA | |
| Cre12.g525650 | SUFS,SUFS1,CSD4 | (1 of 1) K11717 - cysteine desulfurase / selenocysteine lyase (sufS); Group II cysteine desulfurase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAACATCAGACGAGTCAGAAGACCACCCGTATCCAGGGAAATGCCCCA |
| Internal bar code: | GGGTCGCACGTGTCGACCGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2084 |
| LEAP-Seq percent confirming: | 97.9592 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGTGGATGAGCAGGGAT |
| Suggested primer 2: | ATAGGGAGGGGAGAGGGAGA |