| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.057982 |
| Chromosome: | chromosome 16 |
| Location: | 3896181 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g689050 | ACX1,ACO1 | Acyl-CoA oxidase/dehydrogenase; (1 of 1) PTHR10909//PTHR10909:SF250 - ELECTRON TRANSPORT OXIDOREDUCTASE // PEROXISOMAL ACYL-COENZYME A OXIDASE 2 | 5'UTR |
| Cre16.g689100 | PUS6 | (1 of 2) 5.4.99.23 - 23S rRNA pseudouridine(1911/1915/1917) synthase; RNA pseudouridine synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAAACGGGGCGCGGCATAACTATACCGGGGCCCCGTGGCACCAACCCGT |
| Internal bar code: | CAGCAGGTCAGTGTTGTTAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2320 |
| LEAP-Seq percent confirming: | 96.9697 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGGCAGTGGAGGGATTGG |
| Suggested primer 2: | CAATACCCGCAGACCCACTT |