| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.057987 |
| Chromosome: | chromosome 1 |
| Location: | 3282977 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g021050 | TIM22A | (1 of 1) K17790 - mitochondrial import inner membrane translocase subunit TIM22 (TIM22); translocase of inner mitochondrial membrane 22 homolog | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGAAGAGGCGAAGACTCGCGTCGCCTTCAGGCTGGGCCGGCTGGCGGA |
| Internal bar code: | AGTCGCGACAGCACGCCAATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3379 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGTTGCGCGCATTGTAA |
| Suggested primer 2: | TCTTTCTGACGCAGGTGGTC |