| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.058012 |
| Chromosome: | chromosome 2 |
| Location: | 8366628 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g142650 | outside_mRNA | ||
| Cre02.g142700 | (1 of 41) PF13920 - Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGAACGGCACCAGCTCGCGACCCATTGGGGGGCACCCGGAGGGTAAAGA |
| Internal bar code: | ATAAGCTATAACGAAGCACGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2666 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGAAGACCAAAAGGCCG |
| Suggested primer 2: | TCACGCGGACAAGCATTTTG |