| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.058021 |
| Chromosome: | chromosome 12 |
| Location: | 1240655 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g490000 | EIF2A-2,EIF2A,EIF2Ab | (1 of 1) K03237 - translation initiation factor 2 subunit 1 (EIF2S1); Eukaryotic translation initiation factor 2 subunit 1, eIF2-alpha subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATTTTCCGTGCTCTCTGTTGAGCCTTACACTCTGGCCGCTGAGTCCGCC |
| Internal bar code: | TGAAATGATTCTTCGTTACACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2031 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGAAGGAGAACAGTGCCAT |
| Suggested primer 2: | TCTGGCTCGCTTTGACCAAT |