| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.058025 |
| Chromosome: | chromosome 3 |
| Location: | 3361029 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g166300 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAACCAAACGGTGTGAAACTGTGCCTGTCGACGTATCCAGAGCCGAGA |
| Internal bar code: | TATAAATTAAGATAATACGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3199 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCAGATTCCAGAGCCCAA |
| Suggested primer 2: | GCGACGAGTAGACACTAGGC |