| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.058029 |
| Chromosome: | chromosome 10 |
| Location: | 3113476 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g441550 | MAM3B | (1 of 5) K16302 - metal transporter CNNM (CNNM); Protein putatively involved in mitochondrial biogenesis | 5'UTR |
| Cre10.g441600 | OPR48 | OctotricoPeptide Repeat protein 48 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAGCGACAAGGTAGGCAGTGGCCACCGCATTAGGCCACCGCATTGGCC |
| Internal bar code: | ATGATTTTACGTGGTGTTATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1161 |
| LEAP-Seq percent confirming: | 68.4211 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTGTTTGCGTTTTTGCGG |
| Suggested primer 2: | CGGTGAGGCAATTGTTGTGG |