Insertion junction: CLIP2.058031_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.058031
Chromosome:chromosome 2
Location:3296663
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre02.g097550 (1 of 1) PTHR12532:SF0 - TRANSCRIPTIONAL REGULATORY PROTEIN HAH1-RELATED 5'UTR
Cre02.g097600 FKM2 DNA methylase in the FkbM family; (1 of 13) PF05050 - Methyltransferase FkbM domain (Methyltransf_21) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGTACTTGTATAAGGCATTACAATTCTTTCACAGTACTGATCTTCTTTG
Internal bar code:GTTGCTAAGAATAAATACAGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:401
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GGCACTCACAGCTGGATGAT
Suggested primer 2:CCCGCTCTGTAACCCTTTGT