Insertion junction: CLIP2.058058_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.058058
Chromosome:chromosome 17
Location:1310102
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g705500 PHC23 Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) 5'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGAGTCAAACGCCGCTGTAGCTGAGCGCCCGCTGCTCACCAGGGCCGCT
Internal bar code:AAGTCACCTTTACCCTCGGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1275
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGGAGTGCGTTGTTGCCATA
Suggested primer 2:ATCCTAGTTGTAGCGACGCG