| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.058059 |
| Chromosome: | chromosome 9 |
| Location: | 148945 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386731 | (1 of 2) K13095 - splicing factor 1 (SF1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGCCCGGCGGCCCGCCACCGCCCTACCCGCCGCCCTACCCACCGCCTG |
| Internal bar code: | TCCTGTGTTAAATTCCCCAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 686 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAATCCCATCGAGGCGTGT |
| Suggested primer 2: | TTGGATTCCTGACCATGGCC |