| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.058073 |
| Chromosome: | chromosome 6 |
| Location: | 3089498 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g275200 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTCCTCCCATGGGGTGACCTGTTTCCTGCTGCAACTGTGGGCAGCCTG |
| Internal bar code: | TTGTTATCGTGTTGACGGGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1899 |
| LEAP-Seq percent confirming: | 86.4865 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTAAGGAGTGAGGAGGGA |
| Suggested primer 2: | CCGTGTTCCTGCAAAGGTTG |