Insertion junction: CLIP2.058087_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.058087
Chromosome:chromosome 1
Location:667102
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre01.g003751 (1 of 6) IPR000595//IPR005821//IPR018490 - Cyclic nucleotide-binding domain // Ion transport domain // Cyclic nucleotide-binding-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAAGTTAACCCCCTAACCCCCTCCTCTCCCTCCCGGGATTGGTTCAAAG
Internal bar code:CGGGCAGTTTTTACATCCGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:72
LEAP-Seq percent confirming:11.1111
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GAACGTTGCGCAAAGACAGT
Suggested primer 2:GGGGTTCGTTGTTTGTTCCG