| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.058090 |
| Chromosome: | chromosome 9 |
| Location: | 681975 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g404550 | (1 of 2) 2.7.11.20 - [Elongation factor 2] kinase / EF2K | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTTGCACGAATCCGTGGCATTGCGAGGCGTTTGTTCCTGACCGTCGCA |
| Internal bar code: | TGAAGGTTTCCCGAGGCGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 486 |
| LEAP-Seq percent confirming: | 90.4762 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAACCCCTTTGCTCCTCG |
| Suggested primer 2: | TACTAGCAGCCACCTCGACT |